LIME (LIME1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot. Immunohistochemistry on paraffin sections.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME
Specificity	This antibody reacts with the cytoplasmic domain of LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	By precipitation methods; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	Lck interacting transmembrane adaptor 1
Database Link	Entrez Gene 54923 Human UniProt ID Q9H400
Background	LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Synonyms	LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
Reference Data

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