MCL1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: 0.5-1.0 µg/ml. This antibody detects a band of approximately 30 kDa and 38 kDa in Human Raji cell lysates. Immunocytochemistry.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 16 amino acid peptide located near the centre of human MCL-1
Specificity	This antibody recognizes an epitope within the central region (IN) of Myeloid cell leukaemia sequence 1 (MCL-1). SP2170P recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.
Formulation	PBS containing 0.02% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ion Exchange Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	myeloid cell leukemia 1
Database Link	Entrez Gene 4170 Human UniProt ID Q07820
Background	MCL-1 is an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours. The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival.
Synonyms	BCL2L3, Bcl-2-like protein 3, mcl1/EAT, EAT, r004
Reference Data

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