RASSF1 (Isoform A) Mouse Monoclonal Antibody [Clone ID: 3F3]

Specifications

Product Data
Clone Name	3F3
Applications	ELISA, FC, IF, WB
Recommend Dilution	The antibody has been tested by - Immunocytochemistry analysis (~ 1/100) - Flow Cytometry (2-5 µg for 1x106cells) - Western blotting (1/500~1/2,000) - ELISA to assure specificity and reactivity.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human RASSF1A (1-340 aa), purified from E. coli.
Specificity	The antibody recognizes Human RASSF1A. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Ras association domain family member 1
Database Link	Entrez Gene 11186 Human UniProt ID Q9NS23
Background	The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.
Synonyms	RASSF1A, RDA32, REH3P21, NORE2A
Reference Data

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