Growth Hormone (GH1) (21-217) Mouse Monoclonal Antibody [Clone ID: g3H5]

Specifications

Product Data
Clone Name	g3H5
Applications	ELISA, IHC, Neutralize, WB
Recommend Dilution	ELISA. Western blot (1/500-1/1,000). Immunohistochemistry on Paraffin sections (10 µg/ml). This GH antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Neutralization.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Growth hormone (27 - 217 aa) purified from E. coli
Specificity	The antibody recognizes Growth Hormone (hGH) at aa 27-217.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	growth hormone 1
Database Link	Entrez Gene 2688 Human UniProt ID P01241
Background	Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22KDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20KDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Synonyms	Pituitary growth hormone, Growth hormone 1, HGH
Reference Data

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