Tubulin (TUBA1B) Rat Monoclonal Antibody [Clone ID: YOL1/34]

Specifications

Product Data
Clone Name	YOL1/34
Applications	ELISA, IF, IHC, IP, R, WB
Recommend Dilution	ELISA: 10 µg/ml as Detecting antibody. Radioimmunoassay. Western blotting. Immunofluorescence. Flow Cytometry (Not tested). Immunohistochemistry on Frozen and Paraffin Sections.
Reactivity	Human, Mammalian, Mouse, Rat, Yeast, Birds, Drosophila
Host	Rat
Clonality	Monoclonal
Immunogen	Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the rat YB2/0 Myeloma cell line.
Specificity	SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P). This antibody is routinely tested in ELISA on Tubulin.
Formulation	PBS State: Purified State: Liquid purified IgG fraction. Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tubulin alpha 1b
Database Link	Entrez Gene 22143 MouseEntrez Gene 500929 RatEntrez Gene 10376 Human UniProt ID P68363
Background	Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
Synonyms	Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection

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