CD45 (CD45R) Rat Monoclonal Antibody [Clone ID: YKIX753.22.2]

Specifications

Product Data
Clone Name	YKIX753.22.2
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Canine thymocytes. Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Specificity	This antibody was clustered as a CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts and recognises a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all our bred mongrels and beagles tested.
Formulation	PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1ml distilled water
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	Leukocyte common antigen, L-CA, PTPRC, T200, CD45R
Reference Data

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