CD4 Rat Monoclonal Antibody [Clone ID: YKIX302.9]

Specifications

Product Data
Clone Name	YKIX302.9
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells or 100 µl whole blood.  Immunohistochemistry on Frozen Sections.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Canine Concanavilin A activated T-cell blasts.
Specificity	This antibody Clone YKIX302.9 recognises the Canine CD4 cell surface antigen. The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro. In the Dog CD4 is expressed by neutrophils as well as by lymphocytes subsets.
Formulation	PBS State: Purified State: Liquid purfied IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P33705
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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