NTAL (LAT2) Mouse Monoclonal Antibody [Clone ID: NAP-03]

Specifications

Product Data
Clone Name	NAP-03
Applications	IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blot: Recommended dilution: 1-2 mg/ml, incubation 60 min. Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, Positive tissue: tonsil, germinal center.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide corresponding to aa 196-212 of human NTAL coupled to KLH
Specificity	The antibody recognizes the defined epitope (aa 196-212) of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography; > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	linker for activation of T-cells family member 2
Database Link	Entrez Gene 7462 Human UniProt ID Q9GZY6
Background	NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Synonyms	LAB, NTAL, WBS15, WBSCR5, HSPC046
Reference Data

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