CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: MEM-75]

Specifications

Product Data
Clone Name	MEM-75
Applications	FC
Recommend Dilution	Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	NALM-6 human pre-B cell line
Specificity	The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
Formulation	PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer Label: APC State: Liquid purified IgG fraction Label: Conjugated with cross-linked Allophycocyanin under optimum conditions
Purification	Size-exclusion chromatography
Conjugation	APC
Storage Condition	Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light.
Gene Name	transferrin receptor
Database Link	Entrez Gene 7037 Human UniProt ID P02786
Background	CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Reference Data

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