CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

CAT#: SM3103P

CD18 (ITGB2) mouse monoclonal antibody, clone MEM-48, Purified

Conjugation: Unconjugated Biotin FITC



Need it in bulk or conjugated?
Get a free quote

CNY 3,140.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name MEM-48
Applications FC, FN, IHC, IP, WB
Recommend Dilution Flow cytometry: 2 µg/ml.
Immunoprecipitation.
Western blot (non-reducing conditions): 2 µg/ml. Positive Control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel).
Immunohistochemistry on paraffin sections: 10 µg/ml. Positive tissue: spleen, microglia.
Immunohistochemistry on frozen sections.
Functional Assays: The antibody induces high-affinity conformation of LFA-1 complex.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Leucocytes of a patient suffering from a LGL-type leukaemia
Specificity The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain).
CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Formulation PBS, pH~7.4 with 15 mM Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration lot specific
Purification Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name integrin subunit beta 2
Background CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18,
Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These
integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms Integrin beta-2, MFI7
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...