CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

Specifications

Product Data
Clone Name	MEM-48
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow cytometry: 2 µg/ml. Immunoprecipitation. Western blot (non-reducing conditions): 2 µg/ml. Positive Control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel). Immunohistochemistry on paraffin sections: 10 µg/ml. Positive tissue: spleen, microglia. Immunohistochemistry on frozen sections. Functional Assays: The antibody induces high-affinity conformation of LFA-1 complex.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes of a patient suffering from a LGL-type leukaemia
Specificity	The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Formulation	PBS, pH~7.4 with 15 mM Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms	Integrin beta-2, MFI7
Reference Data

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