Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: C-43]

Specifications

Product Data
Clone Name	C-43
Applications	FACS, IF, IHC, IP, WB
Recommend Dilution	Western Blot.  Immunoprecipitation. Immunocytochemistry. Flow Cytometry: 1-5 μg/ml, intracellular staining. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended. Positive Tissue: Prostate.
Reactivity	Bovine, Human, Porcine, Rabbit, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeleton preparation from HeLa Human cervix carcinoma cell line.
Specificity	The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa).  Negative Species: Mouse, Rat, Hamster, Chicken and Xenopus.
Formulation	Phosphate buffered saline (PBS), pH~7.4 State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	52.5 kDa
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.  Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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