Cytokeratin 7 (KRT7) (+ KRT17) Mouse Monoclonal Antibody [Clone ID: C-46]

Specifications

Product Data
Clone Name	C-46
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Immunocytochemistry. Western Blot: 1 µg/ml Sample Preparation: Lysis 1% lauryl maltoside fo for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) Positive Control: HeLa Human cell line. Immunohistochemistry on Paraffin Sections: 20 µg/ml. Pretreatment: 0.1% pepsin, 30 min, 37°C. Incubation: 4°C overnight; Chromogen DAB. Positive tissue: Pancreas.
Reactivity	Human, Porcine, Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Specificity	The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).  Negative Species: Mouse, Rat, Rabbit.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Tissue Culture Supernatant (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	keratin 7
Database Link	Entrez Gene 3855 Human UniProt ID P08729
Background	Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Synonyms	KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin
Reference Data
Protein Families	ES Cell Differentiation/IPS

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