Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: C-04]

Specifications

Product Data
Clone Name	C-04
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive Control: Colon, kidney. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (Cat.No SM3044B) and with the antibody DC-10 (Cat.-No SM1360B). Western Blot: 1-2 µg/ml overnight in 4°C Positive Control: HeLa Human cervix carcinoma cell line, MCF-7 Human breast adenocarcinoma cell line. Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Non-reducing conditions. SDS-PAGE (10% separating gel).
Reactivity	Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeleton preparation of epidermal carcinoma cell line A431.
Specificity	The antibody C-04 reacts with Cytokeratin 18 (45 kDa).
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction (> 95% by SDS-PAGE) Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	UniProt ID P05783
Background	Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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