CD7 Mouse Monoclonal Antibody [Clone ID: MEM-186]

Specifications

Product Data
Clone Name	MEM-186
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Flow cytometry: 2 μg/ml. Western blot (non reducing conditions). Immunohistochemistry (paraffin sections): 5 μg/ml, positive tissue: spleen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human acute myelogenous leukaemia cell line KG-1
Specificity	The antibody reacts with CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Precipitation methods and ion exchange chromatography; Purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	CD7 molecule
Database Link	Entrez Gene 924 Human UniProt ID P09564
Background	CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.
Synonyms	GP40, TP41, Leu-9
Reference Data

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