CD47 Mouse Monoclonal Antibody [Clone ID: MEM-122]

Specifications

Product Data
Clone Name	MEM-122
Applications	FC, IHC, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Non-reducing conditions.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	COS-7 (African green monkey) cells.
Specificity	The antibody reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (Thrombospondin Receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	CHT-Chromatography and Precipitation Methods
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD47 molecule
Database Link	Entrez Gene 961 Human UniProt ID Q08722
Background	CD47 is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein a, SIRPa), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Synonyms	MER6, IAP, OA3
Reference Data

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