CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: MEM-28]

Specifications

Product Data
Clone Name	MEM-28
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 1 µg/ml. Immunoprecipitation. Western Blot: 1 µg/ml. Positive Control: JURKAT Human leukemia T-cell lysate, Kg-1a Human leukemia cell lysate. Sample Preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry on Paraffin Sections: No pre-treatment of tissue sections is essential. Immunocytochemistry: 10 μg/ml (paraformaldehyde fixation can be used).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes and T lymphocytes
Specificity	The antibody clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Formulation	Phosphate Buffered Saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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