CD4 Mouse Monoclonal Antibody [Clone ID: MEM-115]

Specifications

Product Data
Clone Name	MEM-115
Applications	FC, FN, IP, WB
Recommend Dilution	Immunoprecipitation. Functional Application: The antibody blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: 3 µg/ml Application Note: Although it has not been tested rigorously, following data suggest that the antibody is a low-affinity antibody: its binding to T cells increases at elevated temperature; monovalent Fab fragments essentially do not bind to T cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes and T lymphocytes
Specificity	The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD4 molecule
Database Link	Entrez Gene 920 Human UniProt ID P01730
Background	CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin  Intracellular ligands: p56Lck  CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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