CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: MEM-97]

Specifications

Product Data
Clone Name	MEM-97
Applications	FC, IP, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity	Human, Porcine, Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Raji human Burkitt's lymphoma cell line
Specificity	This antibody reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Formulation	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A chromatography (purity > 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	membrane spanning 4-domains A1
Database Link	Entrez Gene 931 Human UniProt ID P11836
Background	CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Synonyms	MS4A1, Leu-16, Bp35, B-cell marker
Reference Data

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