CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

Specifications

Product Data
Clone Name	MEM-154
Applications	FC, FN, IP, WB
Recommend Dilution	Flow cytometry: 5-10 μg/ml (MEM-154 does not react with CD16a present on NK cells in many subjects). Positive control: PBL (peripheral blood lymphocytes).  Immunoprecipitation. Western blot: Non-reducing conditions. Functional application: Blocks binding of human IgG to FcgammaRIII.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	This antobody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Low Endotoxin State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	Fc fragment of IgG receptor IIIa
Database Link	Entrez Gene 2214 Human UniProt ID P08637
Background	CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Synonyms	FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data

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