CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

Specifications

Product Data
Clone Name	MEM-154
Applications	FC, FN, IP, WB
Recommend Dilution	Flow Cytometry: 5-10 μg/ml. Positive Control: PBL (peripheral blood lymphocytes). Application Note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation. Western Blotting (Non-reducing conditions). Functional Assay (The antibody blocks binding of Human IgG to FcgammaRIII).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	The antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.
Formulation	Azide free PBS, pH~7.4 preserved by 0.2 µm filter sterilization. State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Affinity Chromatography on Protein-A.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIIa
Database Link	Entrez Gene 2214 Human UniProt ID P08637
Background	CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Synonyms	FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data

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