CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

Specifications

Product Data
Clone Name	MEM-158
Applications	FC
Recommend Dilution	Flow Cytometry analysis of Human blood cells using 10 µl reagent / 100 μl of whole blood or 10e6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Formulation	Tris buffered saline (TBS) Label: APC State: Liquid Ig fraction Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) Preservative: 15 mM sodium azide Label: Cross-linked Allophycocyanin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	APC
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Background	CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
Synonyms	Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data

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