Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: MoBu-1]

Specifications

Product Data
Clone Name	MoBu-1
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry. Flow Cytometry (Use at 1-5 µg/10e6 cells). This antibody is also useful for detecting proliferating cells by Flow Cytometry or Immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody and not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
Host	Mouse
Clonality	Monoclonal
Immunogen	Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice.
Specificity	Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern.
Formulation	PBS containing 0.08% Sodium Azide as a preservative. Label: FITC State: Liquid purified IgG fration.
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Reference Data

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