Cd200 Mouse Monoclonal Antibody [Clone ID: OX-2]

Specifications

Product Data
Clone Name	OX-2
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; We recommend the use of SM294A for use in functional studies. Immunoprecipitation. Immunohistochemistry on frozen sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Specificity	This antibody recognises the CD200 cell surface antigen, also known as OX-2. This product is routinely tested by flow cytometry on rat thymocytes.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Cd200 molecule
Database Link	Entrez Gene 24560 Rat UniProt ID P04218
Background	CD200 is a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells. Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation.
Synonyms	OX-2 membrane glycoprotein, MOX1, MOX2, My033
Reference Data

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