Icam1 Mouse Monoclonal Antibody [Clone ID: 1A29]

Specifications

Product Data
Clone Name	1A29
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10e6 cells in 100 µl. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (Requires target unmasking). Western Blotting.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Ax cells (a HEV derived cell line).
Specificity	This antibody recognises the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. (Etienne, S. et al.) Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend the use of SM286LE for use in functional studies.
Formulation	PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 25464 Rat UniProt ID Q00238
Background	CD54 is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.
Synonyms	ICAM-1
Reference Data

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