Ptprc (CD45RC) Mouse Monoclonal Antibody [Clone ID: OX-22]

Specifications

Product Data
Clone Name	OX-22
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections: 1/100 - 1/1000. Immunohistochemistry on paraffins sections: 1/100 - 1/1000; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	PHA stimulated rat lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Specificity	This antibody recognises CD45RC, the high molecular weight form of the leucocyte common antigen.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 24699 Rat UniProt ID P04157
Background	This antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations,CD4+ CD45RC (Th 1-like) and CD4+ CD45RC low (Th-2 like).
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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