Pecam1 Mouse Monoclonal Antibody [Clone ID: TLD-3A12]

Specifications

Product Data
Clone Name	TLD-3A12
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA. Western blot. Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 106 cells in 100 µl. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/10-1/100. This clone has been reported as suitable for use in IHC on Formalin-Fixed Paraffin-Embedded Sections pre-treated with 0.2M Boric acid, pH 7.0 (Wilson et al. 2007).
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Specificity	This antibody recognizes PECAM-1 (CD31). Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson et al. 2009). SM272LE is recommended for use in Functional Studies. Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from Rhesus Macaque (Maclean et al. 2001).
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet and endothelial cell adhesion molecule 1
Database Link	Entrez Gene 29583 Rat UniProt ID Q3SWT0
Background	CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). CD31 is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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