CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

Specifications

Product Data
Clone Name	OX-42
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of  1/50-1/100 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/50-1/100. Immunoprecipitation. Immunofluorescence.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Specificity	This antibody recognises the equivalent of Human CD11b, the receptor for the iC3b component of complement. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain (Robinson et al., 1986). Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes (Robinson et al., 1986) as well as inhibit myelin binding and uptake (van der Laan et al., 1996). We recommend the use of SM262LE for use in functional studies.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q63001
Background	The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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