CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

Specifications

Product Data
Clone Name	OX-42
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of SM262LE for use in functional studies.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat resident peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO/U myeloma cell line.
Specificity	This antibody recognises the equivalent of human CD11b, the receptor for the iC3b component of complement. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain.
Formulation	Phosphate Buffered Saline Label: FITC State: Liquid purified IgG fraction Containing 0.09% Sodium Azide and 1% BSA. Stabilizer: Sodium Azide 0.09% Preservative: 1% Bovine Serum Albumin Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q63001
Background	The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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