Cd4 (Domain 1) Mouse Monoclonal Antibody [Clone ID: W3/25]

Specifications

Product Data
Clone Name	W3/25
Applications	FC, FN, IHC
Recommend Dilution	Flow Cytometry: Use 10 μl of 1/100-1/200 diluted antibody to label 106 cells in 100 μl. Routinely tested on Rat splenocytes. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: PLP fixation is recommended for optimal results, See Whiteland et al. for details.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Specificity	This antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995). Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Cd4 molecule
Database Link	Entrez Gene 24932 Rat UniProt ID P05540
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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