Factor VIII (F8) Mouse Monoclonal Antibody [Clone ID: RFFVIIIC/8]

Specifications

Product Data
Clone Name	RFFVIIIC/8
Applications	ELISA, R, WB
Recommend Dilution	ELISA: 1/500-1/15000. Western blot: 1/20-1/200. Radioimmunoassays.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Affinity purified Human Factor VIII
Specificity	Clone RFFVIIIC/8 is a very potent coagulation inhibitor. This antibody recognizes an epitope towards the N-terminus of full length Factor VIII. It also recognizes the 210kDa, 90kDa and 40kDa cleavage products. This clone does not cross-react with von Willebrand factor. Negative Species: Mouse, Canine (Dog) and Rat.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	coagulation factor VIII
Database Link	Entrez Gene 2157 Human UniProt ID P00451
Background	Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and crosslinks to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries.
Synonyms	Procoagulant component, Antihemophilic factor, F8C, AHF
Reference Data

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