NCR1 Mouse Monoclonal Antibody [Clone ID: AKS1]

Specifications

Product Data
Clone Name	AKS1
Applications	FC
Recommend Dilution	Flow Cytometry: neat- 1/5; use 10 µl of the suggested working dilution to label 106 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein consisting of the extracellular region of bovine CD335. Spleen cells from immunised Balb/c mice were fused with cells of the NS-0 myeloma cell line.
Specificity	This antibody is specific for CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer Label: PE State: Lyophilised purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	Entrez Gene 369024 Bovine UniProt ID Q863H2
Background	CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is primarily expressed by a subset natural killer (NK) cells. No expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.
Synonyms	NCR1, LY94, NKp46
Reference Data

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