EMR2 (ADGRE2) Mouse Monoclonal Antibody [Clone ID: 2A1]

Specifications

Product Data
Clone Name	2A1
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises EMR2. Clone 2A1 specifically recognises the stalk region of EMR2.
Formulation	PBS, pH 7.4 Label: PE State: Lyophilized purified IgG fraction Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 0.25 ml distilled water.
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.  Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	adhesion G protein-coupled receptor E2
Database Link	Entrez Gene 30817 Human UniProt ID Q9UHX3
Background	EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.
Reference Data

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