Tubulin (TUBA1B) (Tyr-Tubulin) Rat Monoclonal Antibody [Clone ID: YL1/2]

Specifications

Product Data
Clone Name	YL1/2
Applications	ELISA, IF, IHC, IP, R, WB
Recommend Dilution	ELISA: 1/100-1/1000 (This antibody is routinely tested in ELISA on Tubulin) (3). Immunoprecipitation. Radioimmunoassay. Immunofluorescence. Western blotting: Suitable for use as a Loading Control. Immunohistochemistry on Frozen Sections.
Reactivity	Mammalian, Yeast, Birds
Host	Rat
Clonality	Monoclonal
Immunogen	Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
Specificity	This antibody SM2202P recognizes the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).
Formulation	PBS State: Purified State: Liquid purfied IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G from tissue culture supernatant
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tubulin alpha 1b
Database Link	Entrez Gene 10376 Human UniProt ID P68363
Background	Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Synonyms	Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection

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