HLA Class II DR Rat Monoclonal Antibody [Clone ID: YD1/63.4.10]

Specifications

Product Data
Clone Name	YD1/63.4.10
Applications	FC
Recommend Dilution	Flow Cytometry. Immunohistochemistry on Cryostat Sections. Immunohistochemistry on Paraffin-Embedded Sections.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	DAUDI cells. Donor: immunized DA rat spleen cells. Fusion Partner: Y3 Ag1.2.3 rat myeloma
Specificity	This HLA-DR monoclonal antibody recognizes the HLA-DR (MHC class II) antigen.
Formulation	PBS Label: PE State: Liquid purified Ig fraction from bioreactor supernatant Stabilizer: EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	PE
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
Synonyms	HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Note	Protocol: FLow Cytometry Analysis: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-H cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 1.0–0.5 μg* of this antibody per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes be protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.  Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).  Results: Tissue Distribution by Flow Cytometry Analysis: Cell Concentration: 1x106 cells per test.  Antibody Concentration Used: 0.5 μg/106 cells.  Isotypic Control: PE Rat IgG2a.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.