CD11b Rat Monoclonal Antibody [Clone ID: 5C6]

Specifications

Product Data
Clone Name	5C6
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 μl of 1/50-1/100 diluted antibody to label 106 cells in 100 μl. Functional Assays. Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Thioglycollate-elicted Peritoneal Macrophages (TPM). Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line.
Specificity	SM2199LE reacts with complement type 3 receptor (CR3) and precipitates a heterodimer of 165 and 95 kD. Clone 5C6 inhibits adhesion in vitro and inflammatory recruitment in vivo. Clone 5C6 also inhibits delayed hypersensitivity, inflammation and potentiates bacterial infections and inhibits type 1 diabetes. Partially prevents LPS induced myelomonocytic cell recruitment.
Formulation	Phosphate Buffered Saline State: Low Endotoxin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Database Link	UniProt ID P05555
Background	CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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