Thy1 Rat Monoclonal Antibody [Clone ID: FF-10]
CAT#: SM2128PT
Thy1 rat monoclonal antibody, clone FF-10, Purified
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CNY 2,570.00
货期*
4周
规格
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Specifications
Product Data | |
Clone Name | FF-10 |
Applications | FC, IHC, IP, WB |
Recommend Dilution | Flow Cytometry (1/50-1/100). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Western Blot. |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Immunogen | EL-4 cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line. |
Specificity | This antibody recognises the Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognises a 30kD glycoprotein in Immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines. |
Formulation | PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | thymus cell antigen 1, theta |
Database Link | |
Background | CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. |
Synonyms | Thy-1, THY1, CDw90 |
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