CD4 Mouse Monoclonal Antibody [Clone ID: 44.38]

Specifications

Product Data
Clone Name	44.38
Applications	FC, IF, IHC, IP
Recommend Dilution	Immunoprecipiation. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivity	Goat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Fetal thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.
Specificity	This antibody (Clone 44.38) recognizes the ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes. This clone will immunoprecipitate a 56kD band under Reducing Conditions.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P05542
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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