CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: F8-11-13]

Specifications

Product Data
Clone Name	F8-11-13
Applications	FC, IHC
Recommend Dilution	Immunohistology on Paraffin (1/200). Immunohistology on Frozen. Flow Cytometry (1/50-1/100): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human T lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises the human CD45RA cell surface antigen. Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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