CD59 Rat Monoclonal Antibody [Clone ID: YTH53.1]

Specifications

Product Data
Clone Name	YTH53.1
Applications	FC
Recommend Dilution	Flow cytometry: We do not recommend using this antibody in lysed whole blood techniques due to inhibition of staining caused by CD59 expression on erythrocytes.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human Peripheral Blood T cells. Spleen cells from immunised DA rat fused with the Y3/Ag1.2.3 meloma cell line.
Specificity	Clustered at the fourth leucocyte typing workshop to designation CD59. YTH53.1 reacts with CD59 transfectants. It enhances antibody-mediated lysis by homologous complement and reacts with GPI-linked antigen of 18-20kD size which is broadly expressed on human leucocytes and erythrocytes. Putative ligand for CD2.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water
Purification	Ion exchange chromatography
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD59 molecule
Database Link	Entrez Gene 966 Human UniProt ID P13987
Background	CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Synonyms	MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20
Reference Data

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