Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: 12G10]

Specifications

Product Data
Clone Name	12G10
Applications	ELISA, FC, WB
Recommend Dilution	Flow Cytometry: 1/25-1/50 ELISA: 10 µg/ml. Western Blot. Note: Clone 12G10 has been reported for use in Immunoprecipitation. However, due to the nature of the epitope recognised by clone 12G10, we do not recommend this application.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract. Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
Specificity	This antibody recognises the beta1 integrin. This antibody has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of Fibronectin ligand. This antibody also enhances alpha 5-beta 1 - Fibronectin interactions. Does not react with Rat and Mouse.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 1
Database Link	Entrez Gene 3688 Human UniProt ID P05556
Background	Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Synonyms	Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12
Reference Data

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