LRP1 Mouse Monoclonal Antibody [Clone ID: alpha2MRalpha2]

Specifications

Product Data
Clone Name	alpha2MRalpha2
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA (Direct). Western Blot (Non-reducing conditions): A2Mr alpha-2 recognizes CD91 under non-reducing conditions. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Liver tissue. Immunohistochemistry on Paraffin Sections: clone A2Mr alpha-2 has been used extensively for the detection of CD91 by immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified alpha2 macroglobulin receptor. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This CD91 antibody, clone A2Mr alpha-2 recognizes CD91, a type I membrane protein which is also known as the alpha 2 macroglobulin receptor (alpha 2MR). This antibody Clone A2Mr alpha-2 detects an epitope within the LRP515 chain.
Formulation	PBS, pH7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	LDL receptor related protein 1
Database Link	Entrez Gene 4035 Human UniProt ID Q07954
Background	Low density lipoprotein receptor-related protein (also known as low density LRP, LRP1, alpha-2-macroglobulin receptor or Apolipoprotein E receptor) is an endocytotic receptor that is involved both in endocytosis and in phagocytosis of apoptotic cells. It is required for early embryonic development, is involved in cellular lipid homeostasis, and may play a role in APP metabolism, kinase-dependent intracellular signalling, neuronal calcium signalling and neurotransmission. Low density LRP also plays a role in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha-2-macroglobulin), and is involved in the local metabolism of complexes of plasminogen activators and their endogenous ligands. Low density LPR is postulated to be one of the major players in host resistance to HIV. The precursor low density LRP molecule is cleaved post-translationally to form a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515), which remains non-covalently associated with LRP-85. Following cleavage, the intracellular domain (LPRICD) is present in both the cytoplasm and the nucleus.
Synonyms	A2MR, APR, APOER
Reference Data

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