EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: C595]

Specifications

Product Data
Clone Name	C595
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry. ELISA: 1/100-1/1000. Immunofluorescence. Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections but does requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Urinary MUC-1 mucin. Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
Specificity	This antibody recognises CD227, the breast cancer associated mucin encoded by the Muc-1 gene. In normal tissues expression is restricted to specialised glandular epithelial. SM1708 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the Mucin.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mucin 1, cell surface associated
Database Link	Entrez Gene 4582 Human UniProt ID P15941
Background	MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Synonyms	MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3
Reference Data

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