6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: AD1.1.10]

Specifications

Product Data
Clone Name	AD1.1.10
Applications	FC, IF
Recommend Dilution	Immunofluorescence. Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 1x106 cells in 100 µl.
Host	Mouse
Clonality	Monoclonal
Immunogen	PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. This antibody is routinely tested in Western blotting on Histidine tagged recombinant proteins and reacts against all Histidine-tagged proteins so far tested.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Background	6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Reference Data

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