6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: AD1.1.10]

Specifications

Product Data
Clone Name	AD1.1.10
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/1000. Western blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Host	Mouse
Clonality	Monoclonal
Immunogen	PAX6 transcription factor linked to Histidine tag
Specificity	This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing Histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. This antibody is routinely tested in Western blotting on Histidine tagged recombinant proteins and reacts against all Histidine-tagged proteins so far tested.
Formulation	PBS Label: Biotin State: Liquid purified IgG fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Reference Data

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