Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]

Specifications

Product Data
Clone Name	SV5-PK1
Applications	ELISA, FC, IF, IHC, IP, R, WB
Recommend Dilution	ELISA: 1/1000-1/5000. Western Blot: 1/1000-1/5000. Immunoprecipitation. Radioimmunoassays. Immunofluorescence. Flow Cytometry. Immunohistochemistry on Frozen Sections.
Host	Mouse
Clonality	Monoclonal
Immunogen	Paramyxovirus Simian-Virus 5 (SV5)
Specificity	Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labelled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Reference Data

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