Axonal Growth Cones Mouse Monoclonal Antibody [Clone ID: 2G13]

Specifications

Product Data
Clone Name	2G13
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Protein digestion pretreatment of paraffin sections not required. Antigen retrieval using heat treatment prior to staining of paraffin sections not required. Clone 2G13 has also been reported to work in Western Blotting.
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Embryonic chick tectal membranes. Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognizes intracellular 2G13P which is localized to Growth Cones, particularly filopodia and lamellipodies in developing Rat CNS and embryonic neurons in culture. Studies suggest that 2G13P interacts with the filamentous actin cytoskeleton and therefore may be involved in Growth Cone motility (Stettler et al. 1999). Mouse anti Growth Cone antibody, Clone 2G13 has been used for the detection of growth cones by immunohistochemistry and identification of 40S ribosomal protein SA by Western blotting in Chicken and Rat samples (Baloui et al. 2004).
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Subsequent investigation has identified this protein to be 40S ribosomal protein SA, also known as 37 kDa laminin receptor precursor or Laminin receptor 1 (Baloui et al. 2004). 40S ribosomal protein SA is a 296 amino acid ~37kDa membrane, cytoplasmic and nuclear protein required for the assembly and/or stability of the 40S ribosomal subunit. In vertebrate evolution the molecule has acquired a secondary function as a laminin receptor (UniProt: P50890). In growth cones expression is notable particularly in filopodia and lamellipodia in developing rat CNS and embryonic neurons in culture (Stettler et al. 1999). 40S ribosomal protein SA interacts with the filamentous actin cytoskeleton and therefore may be involved in growth cone motility (Stettler et al. 1999).
Synonyms	Growth Cone Marker
Reference Data

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