CD161 (KLRB1) Mouse Monoclonal Antibody [Clone ID: B199.2]

Specifications

Product Data
Clone Name	B199.2
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line.
Specificity	This antibody recognises the CD161 cell surface antigen. Clone B199.2 recognises a similar epitope to the DX9 monoclonal antibody.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction. Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	killer cell lectin like receptor B1
Database Link	Entrez Gene 3820 Human UniProt ID Q12918
Background	Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. CD161 is expressed by almost all NK cells and with a small subset of CD3+ve T cells. CD161 is a homodimeric cell surface protein, comprising two chains of 40-44kD.
Synonyms	HNKR-P1a, CLEC5B, NKRP1A
Reference Data

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