Cd34 Rat Monoclonal Antibody [Clone ID: MEC14.7]

Specifications

Product Data
Clone Name	MEC14.7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/5 diluted antibody to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	T-end. 1, a pMT transformed endothelial cell line.
Specificity	This antibody recognises the Murine CD34 cell surface antigen which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a Neuraminidase sensitive epitope. As in the Human system, CD34 antibodies in the Mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative, 5% Sucrose and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Reconstitute with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein G.
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD34 antigen
Database Link	Entrez Gene 12490 Mouse UniProt ID Q64314
Background	The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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