HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: MEM-G/9]

Specifications

Product Data
Clone Name	MEM-G/9
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human HLA-G refolded with beta2-microglobulin and peptide
Specificity	The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Purified by protein A; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	major histocompatibility complex, class I, G
Database Link	Entrez Gene 3135 Human UniProt ID P17693
Background	HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Synonyms	HLA-6.0, HLAG, MHC class I antigen G
Note	The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
Reference Data

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