CD5 Mouse Monoclonal Antibody [Clone ID: FE1.1B11]

Specifications

Product Data
Clone Name	FE1.1B11
Applications	FC
Recommend Dilution	Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Feline
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises CD5, a 67kD cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.
Formulation	PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	LEU1, LEU-1, Ly-1, Lyt-1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Reference Data

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